Spermatogenesis in male gonads give rise to male gamete spermatozoa. Studying the mechanisms involved in maturation of immature male germ cells into mature haploid spermatozoa will enable researchers to develop in vitro system for spermatogenesis. Artificial maturation of germ cells is highly significant for infertile patients, especially the cohort of pediatric cancer patients. These patients undergo chemotherapy for cancer treatment, prior to chemotherapeutic treatment clinics cryobank their testicular tissue. Cryobanking provides future hope for such patients to use their testicular tissue for developing sperm in vitro when such models are well established and father their own biological children.